Protocols for using Marchantia have been developed and collected into the OpenPlant protocols kit. These can be accessed directly via OpenPlant protocols in protocols.io

These methods Methods for Marchantia tissue culture and transformation and type IIS assembly can also be viewed in the bioRxiv preprint:

SYSTEMATIC TOOLS FOR REPROGRAMMING PLANT GENE EXPRESSION IN A SIMPLE MODEL, MARCHANTIA POLYMORPHA

Susanna Sauret-Güeto, Eftychios Frangedakis, Linda Silvestri, Marius Rebmann, Marta Tomaselli,  Kasey Markel, Mihails Delmans, Anthony West,  Nicola J. Patron,  Jim Haseloff doi: https://doi.org/10.1101/2020.02.29.971002\